The Introduction and Application of Glycopeptides Analysis

October 08 20:55 2018

Schematic diagram of the steps involved in glycopeptides analysis
Creative Proteomics announced the launching of advanced Glycopeptides Analysis

New York – September 2018- Creative Proteomics announced the launching of advanced Glycopeptides Analysis. Creative Proteomics has established reliable analysis method and clear workflow to provide a tailor-made service with rapid analysis procedures and clearly report, which allows us to build an ideal scientific model to speed up your research.

Glycopeptides analysis is used to provide very helpful information about the glycans on one or more proteins. The most attractive feature of this method is its ability to link glycosylation information to exact locations (glycosylation sites) on proteins. Recently, research focused on facilitating aspects of glycopeptide analysis has advanced significantly in the areas of diagnosing or treating disease and also in developing a better understanding of disease progression and pathology. Since it can discover and characterize some novel and interesting molecules, which leads to the development of some new treatment products.

The techniques used to characterize glycopeptides are still emerging. The bottom-up approach is currently regarded as the most common method used in glycopeptides analysis, which typically involves a combination of specific enzymatic proteolysis followed by fractionation of glycopeptides by affinity chromatography or liquid chromatography and eventually glycopeptide analysis by mass spectrometry. The very critical step before acquiring useful data for glycopeptide analysis is Sample Enrichment of glycopeptides. If just one glycoprotein is to be analyzed, recognizing the protein of interest is usually approached by an antibody. Lectin affinity chromatography is used to select for a group of proteins that are glycosylated. Recently, there is a trend by using a very advantageous approach called hydrophilic interaction liquid chromatography (HILIC) for the glycopeptide enrichment.

Glycopeptides analysis normally uses on-line separation and enrichment combine with the off-line separation methods. LC-MS separation of glycopeptides with reverse phase columns is the most widely adopted pre-MS separation method, which is the same popular as HILIC columns approach. Reverse phase columns typically separate the glycopeptides based on the peptide portion, although most of the glycoforms containing the same peptide sequence co-elute. However, this co-elution has its two-sided as it can be an advantage when it comes to data analysis, in which all the glycoforms of a given glycopeptide are easier to find in the data set and it can also be disadvantageous in that abundant glycoforms can suppress the signals of lesser abundant species. HILIC columns give a way to an alternative separation strategy in that the glycoforms interact with the hydrophilic column; In this way, the glycoforms for a given peptide are more readily separated. Graphitized carbon column can be used to separate glycopeptides isomers.

The glycopeptide-based analysis is used to inform researchers about the glycans, which means it can be used for disease prevention, diagnosing and treatment. Numerous applications of Glycopeptide analysis can be listed, for example, it can be done on endogenous compounds to differentiate between a disease state and a healthy state, in which the result can also be used in increasing the understanding of how the same protein, from different species, can have slightly different properties, such as different circulation half-life, or receptor binding affinity, etc. Furthermore, Glycopeptides originating from recombinantly expressed glycoproteins are also worth investigating. These analyses are completed for a variety of treatment areas. For instance, human erythropoietin, an endogenous compound, can be expressed in mammalian cells and is consumed illicitly by athletes in an effort to enhance their performance. Besides, the glycosylation on EPO provides quite a useful means of distinguishing the compounds, which is very important for drug testers. It is because EPO1 is classified as a banned substance that drug testers need to be able to distinguish between recombinant and endogenous forms. In other cases, recombinant proteins are expressed for human and animal consumption for quite legitimate reasons, such as to aid in fertility, as is the case with the follicle-stimulating hormone. In these instances, characterizing glycosylation of recombinantly expressed proteins is one important step is assessing the overall drug product quality. Finally, glycopeptide analysis can even aid in the development of new products, such as in the development of an HIV vaccine. Glycopeptide analysis has been used to extensively compare the properties of numerous HIV envelope proteins under investigation for their potential to elicit a strong immune response against the HIV-1 virus.

About Creative Proteomics:

Creative Proteomics is programming a full range of services to support various proteome-related researches from identification of single proteins to large-scale proteomic studies. The advancement of our proteomics analysis platforms and our specialized employees allows us to provide efficient high throughput and super-sensitivity services. As one of the leading companies in the omics field with over years of experience in omics study, we provide glycomics analysis service customized to your needs and guide you to a successful research model. More information is available at:https://www.creative-proteomics.com/services/glycopeptides-analysis.htm

Contacts:

Melissa George

[email protected]

1-631-619-7922

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Company Name: Creative Proteomics
Contact Person: Melissa George
Email: Send Email
Phone: 1-631-619-7922
Address:45-1 Ramsey Road
City: Shirley
State: New York
Country: United States
Website: https://www.creative-proteomics.com

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